Mouse TIMP1 Recombinant Protein (RPPB2322)
- SKU:
- RPPB2322
- Product Type:
- Recombinant Protein
- Species:
- Mouse
- Uniprot:
- P12032
- Research Area:
- Enzymes
Description
| Product Name: | Mouse TIMP1 Recombinant Protein |
| Product Code: | RPPB2322 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | TIMP1 |
| Synonyms: | Metalloproteinase inhibitor 1, Erythroid-potentiating activity, EPA, TPA-S1, TPA-induced protein, Tissue inhibitor of metalloproteinases 1, TIMP-1, TIMP1. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | TIMP1 Ligand protein solution (0.5mg/ml) contains Phosphate buffered saline (pH7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | CSCAPPHPQT AFCNSDLVIR AKFMGSPEIN ETTLYQRYKI KMTKMLKGFK AVGNAADIRY AYTPVMESLC GYAHKSQNRS EEFLITGRLR NGNLHISACS FLVPWRTLSP AQQRAFSKTY SAGCGVCTVF PCLSIPCKLE SDTHCLWTDQ VLVGSEDYQS RHFACLPRNP GLCTWRSLGA RHHHHHH |
TIMP1 is a member of the TIMP family. TIMP1 is an inducible glycoprotein produced by various cell types. The TIMP1 glycoprotein is a natural inhibitor of the matrix metalloproteinases, which a group of peptidases involved in degradation of the extracellular matrix. TIMP1 binds in a reversible mode to MMPs, with regions in the N-terminal domain binding to the MMP substrate-binding site. On top of its inhibitory function against most of the known MMPs, TIMP1 is able to promote cell proliferation in a broad range of cell types, and may also have an anti-apoptotic role. Furthermore, TIMP1 has erthyroid-potentiating activity via translocation to the nucleus and also inhibits apoptosis in B-cells.The TIMP1 gene is situated within intron 6 of the synapsin I gene and is transcribed in the opposite direction. TIMP1 activity is dependent on the existence of disulfide bonds.TIMP1 transcription is extremely inducible in reaction to many cytokines and hormones.Increased TIMP1 levels are connected with squamous cell laryngeal carcinoma. TIMP1 overexpression is linked to gastric cancer.
TIMP1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (25-205 a.a.) and fused to a 6 aa His Tag at C-terminus containing a total of 187 amino acids and having a molecular mass of 21kDa.TIMP1 Ligand shows multiple bands between 18-28kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TIMP1: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. Belongs to the protease inhibitor I35 (TIMP) family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted Cellular Component: basement membrane; extracellular matrix; extracellular region; extracellular space; proteinaceous extracellular matrix Molecular Function:cytokine activity; enzyme inhibitor activity; growth factor activity; metal ion binding; metalloendopeptidase inhibitor activity; protease binding; protease inhibitor activity Biological Process: cell activation; negative regulation of apoptosis; negative regulation of membrane protein ectodomain proteolysis; negative regulation of metalloenzyme activity; negative regulation of peptidase activity; positive regulation of cell proliferation; response to cytokine stimulus; response to hormone stimulus |
| UniProt Code: | P12032 |
| NCBI GenInfo Identifier: | 135851 |
| NCBI Gene ID: | 21857 |
| NCBI Accession: | P12032.2 |
| UniProt Secondary Accession: | P12032,P20064, Q61720, |
| UniProt Related Accession: | P12032 |
| Molecular Weight: | 22,628 Da |
| NCBI Full Name: | Metalloproteinase inhibitor 1 |
| NCBI Synonym Full Names: | tissue inhibitor of metalloproteinase 1 |
| NCBI Official Symbol: | Timp1�� |
| NCBI Official Synonym Symbols: | EPA; Clgi; Timp; TIMP-1; TPA-S1�� |
| NCBI Protein Information: | metalloproteinase inhibitor 1 |
| UniProt Protein Name: | Metalloproteinase inhibitor 1 |
| UniProt Synonym Protein Names: | Collagenase inhibitor 16C8 fibroblast; Erythroid-potentiating activity; EPA; TPA-S1; TPA-induced protein; Tissue inhibitor of metalloproteinases 1; TIMP-1 |
| Protein Family: | Metalloproteinase inhibitor |
| UniProt Gene Name: | Timp1�� |
| UniProt Entry Name: | TIMP1_MOUSE |
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