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Mouse Thyrotropin Releasing Hormone (TRH) ELISA Kit

SKU:
MODL01334
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q62361
Sensitivity:
2.77pg/mL
Range:
7.812-500pg/mL
ELISA Type:
Competitive
Synonyms:
TRF,Thyrotropin-Releasing Factor,Thyroliberin,Protirelin,Pro-tirelin,Prothyroliberin,TSH-releasing factor
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
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Description

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Mouse Thyrotropin Releasing Hormone (TRH) ELISA Kit

The Mouse Thyrotropin-Releasing Hormone (TRH) ELISA Kit is specially designed for the accurate quantification of TRH levels in mouse serum, plasma, and tissue homogenates. With a high sensitivity and specificity, this kit delivers reliable and reproducible results, making it an ideal tool for various research applications.TRH, also known as thyrotropin-releasing hormone, is a crucial neuropeptide involved in the regulation of thyroid hormone release and pituitary gland function.

Dysregulation of TRH levels has been linked to various neurological and endocrine disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Mouse Thyrotropin-Releasing Hormone (TRH) ELISA Kit offers researchers a reliable and effective method for studying the role of TRH in disease pathogenesis and exploring new avenues for treatment strategies.

Product Name:Mouse Thyrotropin Releasing Hormone (TRH) ELISA Kit
Product Code:MODL01334
Size:96 Assays
Target:TRH
Synonyms:TRF, Thyrotropin-Releasing Factor, Thyroliberin, Protirelin, Pro-tirelin, Prothyroliberin, TSH-releasing factor
Detection Method:Sandwich
Range:7.812-500pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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