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Mouse Thyroid Stimulating Hormone Receptor (TSHR) ELISA Kit

SKU:
MODL01344
Product Type:
ELISA Kit
Sensitivity:
25.6pg/mL
Range:
62.5-4000pg/mL
ELISA Type:
Sandwich
Synonyms:
LGR3,HTSHR-I,Thyrotropin Receptor,TSH Receptor
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

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Mouse Thyroid Stimulating Hormone Receptor (TSHR) ELISA Kit

The Mouse Thyroid Stimulating Hormone Receptor (TSHR) ELISA Kit is a precise assay designed for the accurate measurement of TSHR levels in mouse serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring dependable and reproducible results, making it ideal for a variety of research purposes.The Thyroid Stimulating Hormone Receptor (TSHR) is a critical receptor involved in thyroid function, playing a key role in regulating hormone production and thyroid gland activity.

Dysregulation of TSHR has been linked to thyroid disorders such as hyperthyroidism and hypothyroidism, making it a valuable biomarker for studying thyroid-related conditions and potential therapeutic interventions. Use the Mouse TSHR ELISA Kit to advance your research in the field of thyroid function and disease.

Product Name:Mouse Thyroid Stimulating Hormone Receptor (TSHR) ELISA Kit
Product Code:MODL01344
Size:96 Assays
Target:TSHR
Synonyms:LGR3, HTSHR-I, Thyrotropin Receptor, TSH Receptor
Detection Method:Sandwich
Range:62.5-4000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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