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Mouse Thymus Expressed Chemokine (TECK) ELISA Kit

SKU:
MODL01257
Product Type:
ELISA Kit
Sensitivity:
11.1pg/mL
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

Mouse Thymus Expressed Chemokine (TECK) ELISA Kit

The Mouse Thymus-Expressed Chemokine (TECK) ELISA Kit is specifically designed for the accurate detection of TECK levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.TECK is a chemokine protein that is expressed in the thymus and plays a critical role in the immune response. It functions as a chemoattractant for lymphocytes, particularly T cells, and is involved in regulating immune cell migration and activation.

TECK is important in inflammatory responses and autoimmune diseases, making it a valuable biomarker for studying immune system function and developing potential therapies.With the Mouse Thymus-Expressed Chemokine (TECK) ELISA Kit, researchers can accurately measure TECK levels in biological samples, allowing for in-depth investigation into immune responses and the development of novel treatment strategies.

Product Name:Mouse Thymus Expressed Chemokine (TECK) ELISA Kit
Product Code:MODL01257
Size:96 Assays
Target:TECK
Synonyms:SCYA25, CCL25, Ckb15, Small Inducible Cytokine Subfamily A(Cys-Cys)Member 25, Ck Beta-15, Thymus Expressed Chemokine, TECKvar
Detection Method:Sandwich
Range:31.2-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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