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Mouse Thymidine Phosphorylase (TP) ELISA Kit

SKU:
MODL01315
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q99N42
Sensitivity:
0.142ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
TYMP,PDECGF,ECGF1,MNGIE,TP,HPD-ECGF,TdRPase,Platelet-Derived Endothelial Cell Growth Factor,Gliostatin
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

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Mouse Thymidine Phosphorylase (TP) ELISA Kit

The Mouse Thymidine Phosphorylase (TP) ELISA Kit is a powerful tool for accurately measuring levels of thymidine phosphorylase in mouse samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers consistent and reliable results, making it an invaluable asset for a variety of research studies.Thymidine phosphorylase is a key enzyme involved in DNA synthesis and nucleotide metabolism, playing a crucial role in cell proliferation and differentiation.

Dysregulation of thymidine phosphorylase has been implicated in various diseases, including cancer and inflammatory disorders, highlighting its importance as a potential biomarker and therapeutic target.By using the Mouse Thymidine Phosphorylase (TP) ELISA Kit, researchers can gain valuable insights into the role of thymidine phosphorylase in disease development and progression, paving the way for innovative treatment strategies and advancements in personalized medicine.

Product Name:Mouse Thymidine Phosphorylase (TP) ELISA Kit
Product Code:MODL01315
Size:96 Assays
Target:TP
Synonyms:TYMP, PDECGF, ECGF1, MNGIE, TP, HPD-ECGF, TdRPase, Platelet-Derived Endothelial Cell Growth Factor, Gliostatin
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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