The Mouse TGFb2 (Transforming Growth Factor Beta 2) CLIA Kit is a specialized assay designed for the accurate quantification of TGFb2 levels in mouse samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for various research applications.TGFb2 is a critical cytokine involved in regulating cell growth, differentiation, and immune response.
It plays a key role in inflammation, tissue repair, and fibrosis, making it a valuable biomarker for studying conditions such as cancer, fibrosis, and autoimmune diseases. The Mouse TGFb2 CLIA Kit provides researchers with a powerful tool to investigate the role of TGFb2 in disease progression and to develop potential therapeutic interventions.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TGF-β2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TGF-β2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TGF-β2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TGF-β2. You can calculate the concentration of Mouse TGF-β2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-108
86-102
92-104
Average (%)
100
93
99
1:4
Range (%)
88-102
86-99
85-98
Average (%)
95
93
90
1:8
Range (%)
94-110
87-102
89-103
Average (%)
100
93
94
1:16
Range (%)
102-120
96-109
85-99
Average (%)
109
102
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
100
EDTA plasma (n=5)
92-106
98
Cell culture media (n=5)
91-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.1
60.73
188.31
26.79
65.79
196.66
Standard deviation
2.17
7.24
12.48
2.15
6.79
22.48
C V (%)
8.01
11.92
6.63
8.03
10.32
11.43
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TGF-β2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TGF-β2 in samples. No significant cross-reactivity or interference between Mouse TGF-β2 and analogues was observed.
