The Mouse TFPI (Tissue Factor Pathway Inhibitor) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of TFPI levels in mouse serum, plasma, and tissue homogenates. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.TFPI is an important regulator of the coagulation pathway, inhibiting the activation of tissue factor and preventing excessive blood clotting. Dysregulation of TFPI has been implicated in various diseases, including thrombosis, sepsis, and inflammatory conditions.
Monitoring TFPI levels can provide valuable insights into the pathophysiology of these diseases and aid in the development of potential therapeutic interventions.With its superior performance and user-friendly format, the Mouse TFPI CLIA Kit is a valuable tool for researchers studying coagulation disorders, thrombotic diseases, and inflammation in mouse models. Trust Assay Genie for accurate and dependable measurement of TFPI levels in your experimental samples.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TFPI. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TFPI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TFPI, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TFPI. You can calculate the concentration of Mouse TFPI in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-108
91-101
99-112
Average (%)
99
96
107
1:4
Range (%)
91-104
99-116
95-111
Average (%)
99
106
103
1:8
Range (%)
99-117
95-108
90-103
Average (%)
107
102
95
1:16
Range (%)
100-111
104-118
101-114
Average (%)
105
110
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-107
97
EDTA plasma (n=5)
96-113
104
Cell culture media (n=5)
86-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.78
112.58
460.3
51.38
108.02
494.88
Standard deviation
6.24
10.68
38.07
5.81
10.19
51.42
C V (%)
12.54
9.49
8.27
11.31
9.43
10.39
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TFPI concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TFPI in samples. No significant cross-reactivity or interference between Mouse TFPI and analogues was observed.
