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Mouse Tetraspanin 30 (TSPAN30) ELISA Kit

SKU:
MODL01346
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P41731
Sensitivity:
0.064ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD63,LAMP-3,ME491,MLA1,OMA81H,Melanoma 1 antigen,Granulophysin,Lysosomal-associated membrane protein 3,Ocular melanoma-associated antigen
Reactivity:
Mouse
Sample Type:
Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Tetraspanin 30 (TSPAN30) ELISA Kit

The Mouse Tetraspanin-30 (TSPAN30) ELISA Kit is specifically designed for the sensitive detection of TSPAN30 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high accuracy and reliability, allowing for consistent and reproducible results for various research applications. Tetraspanin-30 is a key protein involved in cell signaling, cell adhesion, and immune response regulation.

Its presence has been linked to various diseases and immune disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Mouse Tetraspanin-30 (TSPAN30) ELISA Kit is an essential tool for researchers looking to investigate the role and significance of TSPAN30 in various biological processes and disease states in mouse models.

Product Name:Mouse Tetraspanin 30 (TSPAN30) ELISA Kit
Product Code:MODL01346
Size:96 Assays
Target:TSPAN30
Synonyms:CD63, LAMP-3, ME491, MLA1, OMA81H, Melanoma 1 antigen, Granulophysin, Lysosomal-associated membrane protein 3, Ocular melanoma-associated antigen
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)91-9995
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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