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Mouse Taste Receptor Type 1 Member 2 (TAS1R2) ELISA Kit

SKU:
MODL01253
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q925I4
Sensitivity:
0.043ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
TR2,GPR71,T1R2,G Protein-Coupled Receptor 71,Sweet taste receptor T1R2
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

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Mouse Taste Receptor Type 1 Member 2 (TAS1R2) ELISA Kit

The Mouse Taste Receptor Type 1 Member 2 (TAS1R2) ELISA Kit is specifically designed for the accurate detection of TAS1R2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.TAS1R2 is a key component of taste perception in mice, playing a crucial role in detecting sweet tastes. Studying TAS1R2 levels can provide valuable insights into taste preferences, feeding behaviors, and metabolic processes in mice.

This ELISA kit is essential for researchers studying taste receptor function, food intake regulation, and the impact of taste perception on overall health and wellbeing.With its easy-to-use format and high-quality components, the Mouse TAS1R2 ELISA Kit is a valuable tool for advancing research in taste perception and related fields. Order now to accurately measure TAS1R2 levels and uncover new discoveries in taste biology.

Product Name:Mouse Taste Receptor Type 1 Member 2 (TAS1R2) ELISA Kit
Product Code:MODL01253
Size:96 Assays
Target:TAS1R2
Synonyms:TR2, GPR71, T1R2, G Protein-Coupled Receptor 71, Sweet taste receptor T1R2
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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