The Mouse SYP (Synaptophysin) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of synaptophysin levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for research applications in neuroscience and neurodegenerative disorders.Synaptophysin is a key protein found in synaptic vesicles, playing a crucial role in neurotransmission and synaptic function. Abnormal levels of synaptophysin have been linked to various neurological conditions such as Alzheimer's disease, Parkinson's disease, and schizophrenia.
The Mouse SYP CLIA Kit offers researchers a valuable tool for studying synaptic dysfunction and developing potential therapeutic strategies for these disorders.Overall, the Mouse SYP CLIA Kit provides researchers with a dependable and accurate method for measuring synaptophysin levels in mouse samples, making it a valuable asset for advancing our understanding of synaptic function and neurodegenerative diseases.
Product Name:
Mouse SYP (Synaptophysin) CLIA Kit
SKU:
MOES00542
Target:
Mouse SYP (Synaptophysin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SYP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SYP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SYP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse SYP. You can calculate the concentration of Mouse SYP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
104-117
100-114
92-108
Average (%)
110
107
99
1:4
Range (%)
100-114
83-98
91-105
Average (%)
107
90
99
1:8
Range (%)
85-96
93-108
86-98
Average (%)
91
99
92
1:16
Range (%)
97-110
102-114
92-108
Average (%)
103
109
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-114
105
EDTA plasma (n=5)
94-108
102
Cell culture media (n=5)
96-114
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
407.71
817.29
3022.9
415.86
894.81
3042.83
Standard deviation
46.19
93.66
235.18
49.36
105.59
190.48
C V (%)
11.33
11.46
7.78
11.87
11.8
6.26
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse SYP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SYP in samples. No significant cross-reactivity or interference between Mouse SYP and analogues was observed.
