The Mouse sVCAM-1 (Soluble Vascular Cell Adhesion Molecule 1) CLIA Kit is a reliable and efficient tool for the quantitative measurement of soluble VCAM-1 levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Soluble VCAM-1 is an important biomarker for inflammation and vascular dysfunction, playing a key role in various pathological conditions such as atherosclerosis, autoimmune diseases, and inflammatory disorders.
By accurately measuring soluble VCAM-1 levels, researchers can gain valuable insights into the mechanisms underlying these diseases and potential therapeutic targets.Overall, the Mouse sVCAM-1 CLIA Kit is an essential tool for studying vascular biology and inflammatory processes in mouse models, providing researchers with valuable data to advance their research in various fields of study. Visit the link for more information.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse sVCAM-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse sVCAM-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse sVCAM-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse sVCAM-1. You can calculate the concentration of Mouse sVCAM-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-112
86-100
89-104
Average (%)
106
91
95
1:4
Range (%)
95-107
90-106
99-115
Average (%)
100
97
106
1:8
Range (%)
90-105
91-106
100-117
Average (%)
97
97
108
1:16
Range (%)
96-110
87-100
96-110
Average (%)
104
92
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-113
107
EDTA plasma (n=5)
99-114
106
Cell culture media (n=5)
97-110
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.31
270.66
923.58
91.89
288.18
892.28
Standard deviation
10.09
29.96
59.29
11.39
22.82
86.46
C V (%)
10.48
11.07
6.42
12.4
7.92
9.69
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse sVCAM-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse sVCAM-1 in samples. No significant cross-reactivity or interference between Mouse sVCAM-1 and analogues was observed.
