Mouse Suppressors Of Cytokine Signaling 3 (SOCS3) ELISA Kit
The Mouse Suppressors of Cytokine Signaling 3 (SOCS3) ELISA Kit is a powerful tool for the accurate measurement of SOCS3 levels in mouse samples including serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.SOCS3 is a key regulator of the immune response and inflammation, playing a critical role in controlling cytokine signaling pathways. Dysregulation of SOCS3 has been implicated in numerous diseases, including autoimmune disorders, cancer, and metabolic diseases.
Thus, measuring SOCS3 levels can provide valuable insights into the pathogenesis of these conditions and aid in the development of targeted therapies.With its user-friendly protocol and excellent performance, the Mouse SOCS3 ELISA Kit is an indispensable tool for researchers studying the role of SOCS3 in various physiological and pathological processes. Order yours today and unlock the potential of your research.
Product Name:
Mouse Suppressors Of Cytokine Signaling 3 (SOCS3) ELISA Kit
Product Code:
MODL01212
Size:
96 Assays
Target:
SOCS3
Synonyms:
ATOD4, CIS3, Cish3, MGC71791, SOCS-3, SSI-3, SSI3, Cytokine-inducible SH2 protein 3, STAT-induced STAT inhibitor 3
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
