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Mouse Superoxide Dismutase 3, Extracellular (SOD3) ELISA Kit (MODL01214)

SKU:
MODL01214
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O09164
Sensitivity:
0.064ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
SOD-3,EC-SOD,Extracellular Superoxide Dismutase Cu-Zn
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649

Description

system_update_altDatasheet

Mouse Superoxide Dismutase 3, Extracellular (SOD3) ELISA Kit

The Mouse Superoxide Dismutase 3 (Extracellular SOD3) ELISA Kit is designed for the accurate detection of SOD3 levels in mouse serum, plasma, and cell culture supernatants. This kit features high sensitivity and specificity, ensuring reliable and reproducible results, making it ideal for a wide range of research applications.SOD3 is an important antioxidant enzyme that plays a crucial role in protecting cells from oxidative stress and maintaining proper cell function.

Abnormal levels of SOD3 have been linked to various diseases, including cardiovascular diseases, neurodegenerative disorders, and inflammation. Therefore, this ELISA kit is a valuable tool for researchers studying these conditions and developing potential therapies.

Product Name:Mouse Superoxide Dismutase 3, Extracellular (SOD3) ELISA Kit
Product Code:MODL01214
Size:96 Assays
Target:SOD3
Synonyms:SOD-3, EC-SOD, Extracellular Superoxide Dismutase Cu-Zn
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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