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Mouse Stromal Cell Derived Factor 1 (SDF1) ELISA Kit

SKU:
MODL01161
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P40224
Sensitivity:
0.059ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CXCL12,PBSF,P-BSF,SCYB12,SDF1-A,SDF1-B,SDF1A,SDF1B,TLSF-A,TLSF-B,TPAR1,Pre-B Cell Growth-Stimulating Factor,Chemokine C-X-C-Motif Ligand 12
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Mouse Stromal Cell Derived Factor 1 (SDF1) ELISA Kit

The Mouse Stromal Cell-Derived Factor-1 (SDF-1) ELISA Kit is a reliable and sensitive tool for the quantitative detection of SDF-1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high specificity and accuracy, ensuring precise and reproducible results for a variety of research applications.SDF-1, also known as CXCL12, is a key chemokine involved in various biological processes such as stem cell homing, immune response, and tissue repair. It plays a crucial role in regulating cell migration and activation, making it a valuable biomarker for studying inflammatory diseases, cancer metastasis, and stem cell therapies.

With the Mouse SDF-1 ELISA Kit, researchers can gain valuable insights into the role of SDF-1 in different physiological and pathological processes, facilitating the development of novel therapeutic strategies and targeted interventions. Get your kit today and unlock the potential of SDF-1 research in mouse models.

Product Name:Mouse Stromal Cell Derived Factor 1 (SDF1) ELISA Kit
Product Code:MODL01161
Size:96 Assays
Target:SDF1
Synonyms:CXCL12, PBSF, P-BSF, SCYB12, SDF1-A, SDF1-B, SDF1A, SDF1B, TLSF-A, TLSF-B, TPAR1, Pre-B Cell Growth-Stimulating Factor, Chemokine C-X-C-Motif Ligand 12
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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