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Mouse STIP1 Homology And U-Box Containing Protein 1 (STUB1) ELISA Kit

SKU:
MODL01239
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9WUD1
Sensitivity:
0.059ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CHIP,HSPABP2,NY-CO-7,SDCCAG7,UBOX1,C Terminus Of HSC70-Interacting Protein,CLL-associated antigen KW-8,E3 ubiquitin-protein ligase CHIP
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
$899
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Description

system_update_altDatasheet

Mouse STIP1 Homology And U-Box Containing Protein 1 (STUB1) ELISA Kit

The Mouse STIP1 (Homology- and U-box containing protein 1, STUB1) ELISA Kit is specially designed to detect STIP1 levels in mouse samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results, making it an excellent choice for a variety of research applications.STIP1 is a protein known for its involvement in protein folding and degradation pathways. It plays a crucial role in maintaining protein homeostasis and proper cellular function.

Dysregulation of STIP1 has been linked to various diseases including neurodegenerative disorders and cancer, making it a valuable biomarker for studying these conditions and potentially developing targeted therapies.Overall, the Mouse STIP1 ELISA Kit offers researchers a powerful tool to investigate the role of STIP1 in health and disease, opening up new possibilities for understanding and treating complex disorders.

Product Name:Mouse STIP1 Homology And U-Box Containing Protein 1 (STUB1) ELISA Kit
Product Code:MODL01239
Size:96 Assays
Target:STUB1
Synonyms:CHIP, HSPABP2, NY-CO-7, SDCCAG7, UBOX1, C Terminus Of HSC70-Interacting Protein, CLL-associated antigen KW-8, E3 ubiquitin-protein ligase CHIP
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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