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Mouse Stem Cell Factor (SCF) ELISA Kit

SKU:
MODL01155
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P20826
Sensitivity:
6.8pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
KITLG,MGF,SF,KL1,Kitl,FPH2,Kit-Ligand,Steel Factor,Mast Cell Growth Factor,Steel factor,Familial Progressive Hyperpigmentation 2
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
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Description

system_update_altDatasheet

Mouse Stem Cell Factor (SCF) ELISA Kit

The Mouse Stem Cell Factor (SCF) ELISA Kit is a reliable and accurate tool for the detection of stem cell factor levels in mouse serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures precise and reproducible results, making it ideal for various research applications.Stem cell factor (SCF) is a key protein that plays a crucial role in the maintenance and differentiation of stem cells, influencing processes such as hematopoiesis and cell growth.

By measuring SCF levels, researchers can gain valuable insights into stem cell function and behavior, aiding in the study of various diseases and potential therapeutic strategies.Overall, the Mouse Stem Cell Factor (SCF) ELISA Kit offers a valuable tool for researchers seeking to explore the role of SCF in stem cell biology and its implications in disease research and therapy development.

Product Name:Mouse Stem Cell Factor (SCF) ELISA Kit
Product Code:MODL01155
Size:96 Assays
Target:SCF
Synonyms:KITLG, MGF, SF, KL1, Kitl, FPH2, Kit-Ligand, Steel Factor, Mast Cell Growth Factor, Steel factor, Familial Progressive Hyperpigmentation 2
Detection Method:Sandwich
Range:15.6-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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