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Mouse Splicing Factor 3B Subunit 3 (SF3B3) ELISA Kit

SKU:
MODL01176
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q921M3
Sensitivity:
0.056ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
RSE1,SAP130,SF3b130,STAF130,Spliceosome-associated protein 130,Pre-mRNA-splicing factor SF3b 130 kDa subunit
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€749
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Description

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Mouse Splicing Factor 3B Subunit 3 (SF3B3) ELISA Kit

The Mouse Splicing Factor 3B Subunit 3 (SF3B3) ELISA Kit is specially designed for the precise measurement of SF3B3 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a wide range of experimental applications.SF3B3 is a critical subunit of the splicing factor 3B complex, playing a vital role in pre-mRNA splicing. Dysregulation of SF3B3 has been implicated in various diseases, including cancer and genetic disorders, making it a valuable target for research and therapeutic development.

With its reliable performance and ease of use, the Mouse SF3B3 ELISA Kit is an indispensable tool for studying splicing regulation and investigating the molecular mechanisms underlying disease pathogenesis. Visit our website to learn more about this innovative kit and its potential applications in your research.

Product Name:Mouse Splicing Factor 3B Subunit 3 (SF3B3) ELISA Kit
Product Code:MODL01176
Size:96 Assays
Target:SF3B3
Synonyms:RSE1, SAP130, SF3b130, STAF130, Spliceosome-associated protein 130, Pre-mRNA-splicing factor SF3b 130 kDa subunit
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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