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Mouse Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1) ELISA Kit (MODL01222)

SKU:
MODL01222
Product Type:
ELISA Kit
Sensitivity:
31ng/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
OF,PU.1,SFPI1,SPI-1,SPI-A,SPI-1 Proto-Oncogene,Hematopoietic Transcription Factor PU.1,31 kDa Transforming Protein
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€749

Description

system_update_altDatasheet

Mouse Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1) ELISA Kit

The Mouse Spleen Focus-Forming Virus Proviral Integration Oncogene (SPI1) ELISA Kit is specifically designed for the precise measurement of SPI1 levels in mouse spleen samples. This kit provides exceptional sensitivity and specificity, guaranteeing consistent and accurate results for various research purposes.SPI1, also known as PU.1, is a key transcription factor involved in the regulation of hematopoietic cell differentiation and function.

Dysregulation of SPI1 expression has been linked to various diseases, including leukemia and other hematological malignancies. By detecting SPI1 levels in mouse spleen tissues, researchers can gain valuable insights into the role of SPI1 in immune response and hematopoiesis, paving the way for potential therapeutic interventions.

Product Name:Mouse Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1) ELISA Kit
Product Code:MODL01222
Size:96 Assays
Target:SPI1
Synonyms:OF, PU.1, SFPI1, SPI-1, SPI-A, SPI-1 Proto-Oncogene, Hematopoietic Transcription Factor PU.1, 31 kDa Transforming Protein
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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