Mouse Solute Carrier Family 30 Member 4 (SLC30A4) ELISA Kit
The Mouse Solute Carrier Family 30 Member 4 (SLC30A4) ELISA Kit is a highly reliable and sensitive tool for the accurate detection of SLC30A4 levels in mouse serum, plasma, and cell culture supernatants. This kit is designed for researchers in the field of molecular biology and medical research who are studying the role of SLC30A4 in various physiological and pathological processes.SLC30A4 is a member of the solute carrier family of proteins that play a crucial role in the transport of metal ions across cellular membranes. Specifically, SLC30A4 is involved in the transport of zinc, which is an essential micronutrient required for various cellular functions.
Dysregulation of zinc transport by SLC30A4 has been linked to conditions such as diabetes, neurodegenerative disorders, and immune dysfunction, making it an important target for research and potential therapeutic interventions.The Mouse SLC30A4 ELISA Kit offers high specificity and sensitivity, ensuring accurate and reproducible results for in vitro experiments. By measuring SLC30A4 levels in biological samples, researchers can gain valuable insights into the role of this protein in health and disease, paving the way for novel therapeutic strategies and diagnostic tools. Order now and advance your research with confidence.
Product Name:
Mouse Solute Carrier Family 30 Member 4 (SLC30A4) ELISA Kit
Product Code:
MODL01196
Size:
96 Assays
Target:
SLC30A4
Synonyms:
ZNT4, Zinc Transporter 4
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
