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Mouse Solute Carrier Family 30, Member 3 (SLC30A3) ELISA Kit

SKU:
MODL01195
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P97441
Sensitivity:
0.114ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
ZNT3,Zinc Transporter 3
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

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Mouse Solute Carrier Family 30, Member 3 (SLC30A3) ELISA Kit

The Mouse SLC30A3 (Solute Carrier Family 30 Member 3) ELISA Kit is specifically designed for the precise measurement of SLC30A3 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.SLC30A3 is a key transporter protein responsible for the regulation of zinc ion homeostasis within cells. Dysregulation of SLC30A3 has been linked to various diseases, including diabetes, Alzheimer's disease, and cancer.

Therefore, monitoring SLC30A3 levels is crucial for understanding the role of zinc ion transport in these conditions and developing potential therapeutic interventions.With its advanced technology and reliable performance, the Mouse SLC30A3 ELISA Kit is an indispensable tool for researchers studying zinc ion homeostasis and its impact on disease pathology. Get your hands on this kit today to unlock new insights into the biological functions of SLC30A3.

Product Name:Mouse Solute Carrier Family 30, Member 3 (SLC30A3) ELISA Kit
Product Code:MODL01195
Size:96 Assays
Target:SLC30A3
Synonyms:ZNT3, Zinc Transporter 3
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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