null

Mouse Sodium/Glucose Cotransporter 1 (SGLT1) ELISA Kit

SKU:
MODL01182
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8C3K6
Sensitivity:
0.061ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
SLC5A1,NAGT,Solute Carrier Family 5 Member 1,High affinity sodium-glucose cotransporter,Na(+)/glucose cotransporter 1
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Sodium/Glucose Cotransporter 1 (SGLT1) ELISA Kit

The Mouse Sodium Glucose Cotransporter 1 (SGLT1) ELISA Kit is specifically designed for the quantitative detection of SGLT1 levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for researchers studying glucose transport mechanisms.SGLT1 is a key protein involved in glucose absorption in the intestines and kidneys, playing a crucial role in maintaining glucose homeostasis.

Dysregulation of SGLT1 has been implicated in metabolic disorders such as diabetes and obesity, making this kit essential for studying these conditions and potential therapeutic interventions.With its easy-to-use protocol and comprehensive instructions, the Mouse SGLT1 ELISA Kit is a valuable tool for scientists working in the fields of metabolism, nutrition, and diabetes research. Get accurate and reproducible results with this advanced kit from Assay Genie.

Product Name:Mouse Sodium/Glucose Cotransporter 1 (SGLT1) ELISA Kit
Product Code:MODL01182
Size:96 Assays
Target:SGLT1
Synonyms:SLC5A1, NAGT, Solute Carrier Family 5 Member 1, High affinity sodium-glucose cotransporter, Na(+)/glucose cotransporter 1
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products