The Mouse SOD1 (Superoxide Dismutase 1) Soluble ELISA Kit is specifically designed for the accurate and reliable detection of soluble SOD1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.SOD1 is a critical antioxidant enzyme that plays a key role in neutralizing free radicals and protecting cells from oxidative damage. Imbalances in SOD1 levels have been linked to various diseases such as amyotrophic lateral sclerosis (ALS) and other neurodegenerative disorders.
As a result, measuring SOD1 levels using this ELISA kit can provide valuable insights into disease mechanisms and potential therapeutic interventions.Overall, the Mouse SOD1 Soluble ELISA Kit offers researchers a powerful tool for studying oxidative stress, neurodegeneration, and other related conditions in mouse models, ultimately advancing our understanding of these complex biological processes.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with SOD1. During the reaction, SOD1 in the sample or standard competes with a fixed amount of SOD1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to SOD1. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SOD1 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
95-108
94-110
Average (%)
101
102
100
1:4
Range (%)
85-97
92-105
98-111
Average (%)
90
97
103
1:8
Range (%)
87-99
89-102
99-115
Average (%)
92
97
106
1:16
Range (%)
84-98
93-107
98-112
Average (%)
91
99
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
100
EDTA plasma (n=5)
89-101
95
Cell culture media (n=5)
90-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.5
3.6
0.5
1.4
3.3
Standard deviation
0.03
0.08
0.19
0.03
0.07
0.11
C V (%)
6
5.33
5.28
6.0
5.0
3.33
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of SOD1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes SOD1 in samples. No significant cross-reactivity or interference between SOD1 and analogues was observed.
