Mouse Slit Homolog 2 (Slit2) ELISA Kit
- SKU:
- MODL01201
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- Q9R1B9
- Sensitivity:
- 34pg/mL
- Range:
- 78.125-5000pg/mL
- ELISA Type:
- Sandwich
- Synonyms:
- SLIL3
- Reactivity:
- Mouse
- Sample Type:
- Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
Frequently bought together:
Description
system_update_altDatasheet
Mouse Slit Homolog 2 (Slit2) ELISA Kit
The Mouse SLIT Homolog 2 (SLIT2) ELISA Kit is specifically designed for the accurate detection of SLIT2 levels in mouse serum, plasma, and cell culture supernatants. With a focus on high sensitivity and specificity, this kit provides reliable and reproducible results, making it an ideal tool for a variety of research applications.SLIT2 is a critical protein involved in axon guidance and cell migration, playing a key role in nervous system development and functioning.
Dysregulation of SLIT2 has been linked to various neurological disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Mouse SLIT Homolog 2 (SLIT2) ELISA Kit is a versatile and essential tool for researchers looking to investigate the role of SLIT2 in biological processes and disease pathology in mouse models.
| Product Name: | Mouse Slit Homolog 2 (Slit2) ELISA Kit |
| Product Code: | MODL01201 |
| Size: | 96 Assays |
| Target: | Slit2 |
| Synonyms: | SLIL3 |
| Detection Method: | Sandwich |
| Range: | 78.1-5,000pg/mL |
| Shelf Life: | 12 months |
| Note: | For research use only |
| Recovery: | Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples. | ||||||||||||||||||||
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| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. | ||||||||||||||||||||
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| Intra-assay Precision: | Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively. | ||||||||||||||||||||
| Inter-assay Precision: | Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate. | ||||||||||||||||||||
| Stability: | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end. |
| Step | Protocol |
| 1. | Prepare all reagents, samples and standards |
| 2. | Add 100µL standard or sample to each well. Incubate 2 hours at 37°C |
| 3. | Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C |
| 4. | Aspirate and wash 3 times |
| 5. | Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C |
| 6. | Aspirate and wash 5 times |
| 7. | Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C |
| 8. | Add 50µL Stop Solution. Read at 450nm immediately. |
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