The Mouse SIGA (Secretory Immunoglobulin A) Elisa Kit is a highly reliable and accurate tool for detecting SIGA levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it perfect for various research applications.SIGA is a key component of the immune system, playing a crucial role in mucosal immunity and protecting against infections in the respiratory and gastrointestinal tracts.
Abnormal levels of SIGA have been associated with conditions such as autoimmune diseases, allergies, and infectious diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Mouse SIGA Elisa Kit offers researchers a powerful tool to study SIGA levels in mouse models, furthering our understanding of immune responses and potential treatment options for a variety of diseases.
Product Name:
Mouse sIgA (Secretory Immunoglobulin A) ELISA Kit
SKU:
MOES01461
Target:
Mouse sIgA (Secretory Immunoglobulin A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse sIgA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse sIgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse sIgA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse sIgA. You can calculate the concentration of Mouse sIgA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-112
90-103
91-103
Average (%)
103
97
97
1:4
Range (%)
87-99
84-94
83-94
Average (%)
94
89
89
1:8
Range (%)
89-99
86-100
84-98
Average (%)
94
92
89
1:16
Range (%)
89-101
84-95
86-99
Average (%)
95
90
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
95
EDTA plasma (n=5)
88-101
94
Cell culture media (n=5)
87-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.51
1.17
4.34
0.46
1.16
4.6
Standard deviation
0.03
0.06
0.24
0.03
0.06
0.18
C V (%)
5.88
5.13
5.53
6.52
5.17
3.91
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse sIgA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse sIgA in samples. No significant cross-reactivity or interference between Mouse sIgA and analogues was observed.
