The Mouse SHBG (Sex Hormone Binding Globulin) ELISA Kit is specifically designed for the accurate measurement of SHBG levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing reliable and reproducible results that are ideal for a variety of research applications.SHBG is a critical protein that binds to sex hormones such as testosterone and estrogen, regulating their bioavailability and influencing their physiological effects.
Understanding SHBG levels is essential for studying conditions related to hormonal imbalances, reproductive health, and metabolic disorders.By using the Mouse SHBG ELISA Kit, researchers can effectively investigate the role of SHBG in various biological processes and diseases, ultimately leading to a better understanding of hormone regulation and the development of potential therapeutic strategies.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SHBG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SHBG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SHBG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse SHBG. You can calculate the concentration of Mouse SHBG in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
92-104
93-108
Average (%)
93
97
99
1:4
Range (%)
87-100
81-93
85-94
Average (%)
94
88
89
1:8
Range (%)
89-104
82-95
88-99
Average (%)
95
87
93
1:16
Range (%)
94-106
84-97
84-97
Average (%)
99
91
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
97
EDTA plasma (n=5)
90-105
96
Cell culture media (n=5)
90-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100
273.63
767.14
104.15
266.24
693.02
Standard deviation
5.67
12.09
23.01
5.45
10.73
28.55
C V (%)
5.67
4.42
3.0
5.23
4.03
4.12
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse SHBG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SHBG in samples. No significant cross-reactivity or interference between Mouse SHBG and analogues was observed.
