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Mouse Serpin F1 / PEDF ELISA Kit

SKU:
MOFI01040
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P97298
Sensitivity:
0.094ng/ml
Range:
0.156-10ng/ml
ELISA Type:
Sandwich
Synonyms:
PEDF, Pigment Epithelium Derived Factor, Serpin F1, Pigment epithelium-derived factor, Cell prolifeRation-inducing gene 35 protein, EPC-1
Reactivity:
Mouse
€649
Frequently bought together:

Description

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Mouse Serpin F1/PEDF ELISA Kit

The Mouse Serpin F1 (PEDF) ELISA Kit is specifically designed for the precise measurement of Serpin F1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results for various research purposes.Serpin F1, also known as pigment epithelium-derived factor (PEDF), plays a vital role in regulating angiogenesis, cell growth, and survival. Its dysregulation has been linked to various diseases, including cancer, diabetes, and neurodegenerative disorders.

By quantifying Serpin F1 levels, researchers can gain valuable insights into disease mechanisms and potentially identify new therapeutic targets.Overall, the Mouse Serpin F1 (PEDF) ELISA Kit is a valuable tool for studying the role of Serpin F1 in physiological and pathological processes, ultimately advancing our understanding of complex diseases and aiding in the development of novel treatment strategies.

Product Name:Mouse Serpin F1 / PEDF ELISA Kit
Product Code:MOFI01040
Size:96 Assays
Alias:PEDF, Pigment Epithelium Derived Factor, Serpin F1, Pigment epithelium-derived factor, Cell prolifeRation-inducing gene 35 protein, EPC-1
Detection Method:Sandwich ELISA
Application:This immunoassay kit allows for the in vitro quantitative determination of Mouse PEDF concentrations in serum plasma and other biological fluids.
Sensitivity:0.094ng/ml
Range:0.156-10ng/ml
Storage:4°C for 6 months
Note:For Research Use Only
Recovery: Matrices listed below were spiked with certain level of Mouse PEDF and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse PEDF in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)89-10495
EDTA plasma(n=5)87-10495
UFH plasma(n=5)87-10294
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse PEDF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:8
serum(n=5)87-104%91-105%90-96%
EDTA plasma(n=5)90-95%89-98%85-101%
UFH plasma(n=5)80-94%80-98%87-97%
Intra Assay:CV <8%
Inter Assay:CV <10%
ComponentQuantityStorage
ELISA Microplate (Dismountable)8×12 strips4°C for 6 months
Lyophilized Standard24°C/-20°C
Sample/Standard Dilution Buffer20ml4°C
Biotin-labeled Antibody(Concentrated)120ul4°C (Protect from light)
Antibody Dilution Buffer10ml4°C
HRP-Streptavidin Conjugate(SABC)120ul4°C (Protect from light)
SABC Dilution Buffer10ml4°C
TMB Substrate10ml4°C (Protect from light)
Stop Solution10ml4°C
Wash Buffer(25X)30ml4°C
Plate Sealer5-

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir
UniprotP97298
UniProt Protein Function:PEDF: a secreted neurotrophic protein that induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. Extracellular phosphorylation enhances antiangiogenic activity.
UniProt Protein Details:

Protein type:Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11|11 B5

Cellular Component: axon; axon hillock; basement membrane; cell soma; extracellular matrix; extracellular region; extracellular space; perinuclear region of cytoplasm

Molecular Function:serine-type endopeptidase inhibitor activity

Biological Process: negative regulation of angiogenesis; negative regulation of inflammatory response; positive regulation of neurogenesis; short-term memory

UniProt Code:P97298
NCBI GenInfo Identifier:117606335
NCBI Gene ID:20317
NCBI Accession:NP_035470.3
UniProt Secondary Accession:P97298,O70629, O88691,
UniProt Related Accession:P97298
Molecular Weight:46,234 Da
NCBI Full Name:pigment epithelium-derived factor
NCBI Synonym Full Names:serine (or cysteine) peptidase inhibitor, clade F, member 1
NCBI Official Symbol:Serpinf1  
NCBI Official Synonym Symbols:Pedf; Sdf3; EPC-1; Pedfl; AI195227  
NCBI Protein Information:pigment epithelium-derived factor
UniProt Protein Name:Pigment epithelium-derived factor
UniProt Synonym Protein Names:Caspin; Serpin F1; Stromal cell-derived factor 3; SDF-3
UniProt Gene Name:Serpinf1  

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1. Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells!
2.Aliquot 0.1ml standard solutions into the standard wells.
3. Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well.
4. Add 0.1 ml of properly diluted sample (Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells.
5.Seal the plate with a cover and incubate at 37 °C for 90 min.
6. Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2.
7. Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall.
8.Seal the plate with a cover and incubate at 37°C for 60 min.
9. Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash.
10. Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min.
11. Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min.
12. Add 90 µL of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color.
13. Add 50 µL of Stop solution into each well and mix thoroughly. The color changes into yellow immediately.
14. Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample Type Protocol
Serum:

If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

Plasma:

Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.

Urine & Cerebrospinal Fluid:

Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.

Cell culture supernatant:

Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.

Cell lysates:

Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20°C.

Tissue homogenates:

The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.

Tissue lysates:

Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.

Breast Milk:

Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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