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Mouse Serotonin Transporter (SERT) ELISA Kit

SKU:
MODL01172
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q60857
Sensitivity:
0.055ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
SLC6A4,SLC6-A4,HTT,5-HTT,5HTT,OCD1,hSERT,Solute Carrier Family 6,Member 4,Neurotransmitter Transporter,Serotonin,Sodium-dependent serotonin transporter
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

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Mouse Serotonin Transporter (SERT) ELISA Kit

The Mouse Serotonin Transporter (SERT) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of serotonin transporter levels in mouse samples. This kit is ideal for researchers studying neurotransmission, mood disorders, and neurological diseases.The serotonin transporter, also known as SERT, plays a critical role in the reuptake of serotonin in the brain, affecting mood regulation and emotional processing.

Dysregulation of SERT has been implicated in conditions such as depression, anxiety, and schizophrenia, highlighting the importance of studying this protein in research.With its reliable performance and reproducible results, the Mouse SERT ELISA Kit from AssayGenie is a valuable tool for investigating the role of serotonin transporter in various physiological and pathological processes.

Product Name:Mouse Serotonin Transporter (SERT) ELISA Kit
Product Code:MODL01172
Size:96 Assays
Target:SERT
Synonyms:SLC6A4, SLC6-A4, HTT, 5-HTT, 5HTT, OCD1, hSERT, Solute Carrier Family 6,Member 4, Neurotransmitter Transporter,Serotonin, Sodium-dependent serotonin transporter
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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