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Mouse Serine Peptidase Inhibitor Kazal Type 5 (SPINK5) ELISA Kit

SKU:
MODL01223
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q148R4
Sensitivity:
28pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
LEKTI,LETKI,NETS,NS,VAKTI,Lymphoepithelial Kazal-Type-Related Inhibitor,Lympho-epithelial Kazal-type-related inhibitor
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
$779
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Description

system_update_altDatasheet

Mouse Serine Peptidase Inhibitor Kazal Type 5 (SPINK5) ELISA Kit

The Mouse Serine Peptidase Inhibitor, Kazal Type 5 (SPINK5) ELISA Kit is specially designed for the precise measurement of SPINK5 levels in mouse serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.SPINK5 is a vital protein that plays a critical role in skin barrier function and regulation of inflammation. Dysregulation of SPINK5 has been associated with conditions such as atopic dermatitis and other skin disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.

Utilizing the Mouse SPINK5 ELISA Kit, researchers can confidently measure SPINK5 levels in mouse samples, providing valuable insights into the pathophysiology of skin diseases and advancing our understanding of potential treatment options.

Product Name:Mouse Serine Peptidase Inhibitor Kazal Type 5 (SPINK5) ELISA Kit
Product Code:MODL01223
Size:96 Assays
Target:SPINK5
Synonyms:LEKTI, LETKI, NETS, NS, VAKTI, Lymphoepithelial Kazal-Type-Related Inhibitor, Lympho-epithelial Kazal-type-related inhibitor
Detection Method:Sandwich
Range:78.125-5,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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