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Mouse Serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2) ELISA Kit

SKU:
MODL01228
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P97363
Sensitivity:
0.055ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
LCB2,SPT2,Long chain base biosynthesis protein 2,Serine-palmitoyl-CoA transferase 2
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Mouse Serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2) ELISA Kit

The Mouse Serine Palmitoyltransferase Long Chain Base Subunit 2 (SPTLC2) ELISA Kit is a powerful tool for the precise measurement of SPTLC2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.SPTLC2 is a key enzyme in the sphingolipid biosynthesis pathway, playing a critical role in maintaining cellular homeostasis and lipid metabolism. Dysregulation of SPTLC2 has been linked to various diseases, including metabolic disorders, neurodegenerative conditions, and cancer.

By accurately quantifying SPTLC2 levels, researchers can gain valuable insights into disease mechanisms and develop targeted therapeutic strategies.Overall, the Mouse SPTLC2 ELISA Kit provides a reliable and efficient solution for studying the role of SPTLC2 in physiological and pathological processes, making it an indispensable tool for biomedical research.

Product Name:Mouse Serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2) ELISA Kit
Product Code:MODL01228
Size:96 Assays
Target:SPTLC2
Synonyms:LCB2, SPT2, Long chain base biosynthesis protein 2, Serine-palmitoyl-CoA transferase 2
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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