The Mouse sSELL (L-selectin) CLIA Kit (MOES00341) is a highly reliable and sensitive assay designed for the accurate and precise quantification of sSELL (L-selectin) levels in mouse serum, plasma, and cell culture supernatants. This kit offers high specificity, ensuring dependable and consistent results, making it an excellent tool for a variety of research applications.sSELL (L-selectin) is an important adhesion molecule involved in mediating leukocyte rolling and homing to sites of inflammation.
Its expression is crucial in immune response and inflammatory processes, making it a valuable biomarker for studying these pathways and potential therapeutic interventions.With the Mouse sSELL (L-selectin) CLIA Kit (MOES00341), researchers can gain valuable insights into immune responses, inflammation, and related pathways in mouse models, ultimately advancing our understanding of various diseases and aiding in the development of novel treatments.
Product Name:
Mouse SELL (L-Selectin) CLIA Kit
SKU:
MOES00341
Target:
Mouse SELL (L-Selectin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SELL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SELL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SELL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse SELL. You can calculate the concentration of Mouse SELL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
88-102
93-106
Average (%)
97
95
99
1:4
Range (%)
94-110
84-98
83-99
Average (%)
101
91
90
1:8
Range (%)
102-118
94-110
101-117
Average (%)
109
100
107
1:16
Range (%)
86-97
100-116
100-114
Average (%)
91
108
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
101
EDTA plasma (n=5)
93-106
99
Cell culture media (n=5)
91-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
102.1
183.42
901.12
109.05
200.9
960.74
Standard deviation
11.03
14.55
67.67
9.87
23.36
108.95
C V (%)
10.8
7.93
7.51
9.05
11.63
11.34
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse SELL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SELL in samples. No significant cross-reactivity or interference between Mouse SELL and analogues was observed.
