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Mouse Selectin, Platelet (SELP) ELISA Kit

SKU:
MODL01168
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q01102
Sensitivity:
0.068ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD62P,P-Selectin,PSEL,GMP140,LECAM3,GRMP,GMRP,PADGEM,Leukocyte-endothelial cell adhesion molecule 3,Platelet activation dependent granule-external membrane
Reactivity:
Mouse
Sample Type:
Serum, Platelet-Poor Plasma, Tissue Homogenates, Cell Culture Supernatants And Other Biological Fluids
€599
Frequently bought together:

Description

system_update_altDatasheet

Mouse Selectin, Platelet (SELP) ELISA Kit

The Mouse Selectin Platelet SELP ELISA Kit is a reliable and accurate tool for detecting levels of selectin platelet (SELP) in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Selectin platelet is a key protein involved in the process of platelet activation and adhesion, playing a crucial role in inflammation and thrombosis. Monitoring levels of SELP can provide valuable insights into the mechanisms of these processes, making this ELISA kit essential for studying inflammatory diseases, cardiovascular disorders, and other related conditions.

With its user-friendly protocol and high-performance characteristics, the Mouse Selectin Platelet SELP ELISA Kit is an invaluable tool for researchers seeking to deepen their understanding of platelet function and explore potential therapeutic targets.

Product Name:Mouse Selectin, Platelet (SELP) ELISA Kit
Product Code:MODL01168
Size:96 Assays
Target:SELP
Synonyms:CD62P, P-Selectin, PSEL, GMP140, LECAM3, GRMP, GMRP, PADGEM, Leukocyte-endothelial cell adhesion molecule 3, Platelet activation dependent granule-external membrane
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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