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Mouse Secretory Leukocyte Peptidase Inhibitor (SLPI) ELISA Kit

SKU:
MODL01203
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P97430
Sensitivity:
35pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
ALK1,ALP,MPI,BLPI,HUSI,HUSI-I,WAP4,WFDC4,Antileukoproteinase,Seminal proteinase inhibitor,Mucus proteinase inhibitor,WAP four-disulfide core domain protein 4
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Secretory Leukocyte Peptidase Inhibitor (SLPI) ELISA Kit

The Mouse Secretory Leukocyte Peptidase Inhibitor (SLPI) ELISA Kit is a reliable tool for the detection of SLPI levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.SLPI is a key inhibitor of proteases involved in inflammatory processes and immune response regulation. It plays a critical role in protecting the respiratory epithelium and is implicated in various lung diseases such as COPD and asthma.

Detecting SLPI levels can provide valuable insights into these conditions and aid in the development of potential therapeutic strategies.Overall, the Mouse SLPI ELISA Kit is a valuable tool for researchers studying inflammatory and respiratory diseases in mouse models. Its precision and versatility make it an essential component of any research focusing on SLPI and its related pathways.

Product Name:Mouse Secretory Leukocyte Peptidase Inhibitor (SLPI) ELISA Kit
Product Code:MODL01203
Size:96 Assays
Target:SLPI
Synonyms:ALK1, ALP, MPI, BLPI, HUSI, HUSI-I, WAP4, WFDC4, Antileukoproteinase, Seminal proteinase inhibitor, Mucus proteinase inhibitor, WAP four-disulfide core domain protein 4
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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