The Mouse Secretory Immunoglobulin A (sIgA) ELISA Kit is a reliable tool for the quantification of sIgA levels in mouse serum, plasma, and other biological samples. With its high sensitivity and specificity, this kit ensures precise and accurate results for your research needs.sIgA is an important component of the immune system, primarily found in mucosal secretions such as saliva and tears. It plays a crucial role in protecting the body against pathogens and maintaining immune homeostasis at mucosal surfaces. Monitoring sIgA levels can provide valuable insights into the immune response and help researchers better understand infectious diseases, autoimmune disorders, and other conditions related to mucosal immunity.
Whether you are studying the impact of dietary interventions, investigating the effects of stress on the immune system, or exploring the role of sIgA in chronic inflammatory conditions, the Mouse Secretory Immunoglobulin A (sIgA) ELISA Kit is an essential tool for your laboratory research. Trust in its reliability and accuracy to advance your scientific endeavors.
Product Name:
Mouse Secretory Immunoglobulin A (sIgA) ELISA Kit
Product Code:
MODL01187
Size:
96 Assays
Target:
sIgA
Synonyms:
S-IgA
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
85-103
94
Heparin plasma (n=5)
87-99
93
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
