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Mouse Secreted Frizzled Related Protein 4 (SFRP4) ELISA Kit

SKU:
MODL01179
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9Z1N6
Sensitivity:
0.065ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
FRP-4,FRPHE,Frizzled protein, human endometrium
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
$779
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Description

system_update_altDatasheet

Mouse Secreted Frizzled Related Protein 4 (SFRP4) ELISA Kit

The Mouse Secreted Frizzled-Related Protein 4 (SFRP4) ELISA Kit is specifically designed for the quantitative detection of SFRP4 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.SFRP4 is a critical protein that belongs to the secreted frizzled-related protein family and is involved in the regulation of Wnt signaling pathways.

Dysregulation of SFRP4 has been linked to various diseases, including cancer, diabetes, and cardiovascular disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Mouse SFRP4 ELISA Kit provides researchers with a reliable tool to investigate the role of SFRP4 in disease pathogenesis and explore its potential as a target for novel treatment strategies.

Product Name:Mouse Secreted Frizzled Related Protein 4 (SFRP4) ELISA Kit
Product Code:MODL01179
Size:96 Assays
Target:SFRP4
Synonyms:FRP-4, FRPHE, Frizzled protein, human endometrium
Detection Method:Sandwich
Range:62.5-4,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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