The Mouse SDC4 (Syndecan-4) CLIA Kit is specifically designed for the accurate detection of Syndecan-4 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.Syndecan-4 is a key cell surface proteoglycan that plays a critical role in cell adhesion, migration, and signaling processes. It is involved in various physiological and pathological conditions, including wound healing, inflammation, and cancer progression.
Measuring Syndecan-4 levels can provide valuable insights into these processes and aid in the development of potential therapeutic strategies.Overall, the Mouse SDC4 (Syndecan-4) CLIA Kit is a valuable tool for researchers looking to study the role of Syndecan-4 in various biological processes and diseases, offering a reliable and efficient method for detecting and quantifying Syndecan-4 levels in mouse samples.
Product Name:
Mouse SDC4 (Syndecan 4) CLIA Kit
SKU:
MOES00544
Target:
Mouse SDC4 (Syndecan 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SDC4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SDC4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SDC4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse SDC4. You can calculate the concentration of Mouse SDC4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-103
103-115
97-113
Average (%)
95
108
103
1:4
Range (%)
94-106
88-101
90-104
Average (%)
100
94
97
1:8
Range (%)
92-107
90-101
95-107
Average (%)
99
96
100
1:16
Range (%)
91-105
99-111
104-121
Average (%)
97
105
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
97
EDTA plasma (n=5)
85-98
92
Cell culture media (n=5)
97-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
220.49
624.11
1575.41
232.09
635.27
1730.09
Standard deviation
19.78
52.43
99.57
28.59
60.16
116.44
C V (%)
8.97
8.4
6.32
12.32
9.47
6.73
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse SDC4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SDC4 in samples. No significant cross-reactivity or interference between Mouse SDC4 and analogues was observed.
