Mouse sCD40L Recombinant Protein (RPPB0895)
- SKU:
- RPPB0895
- Product Type:
- Recombinant Protein
- Species:
- Mouse
- Uniprot:
- P27548
- Research Area:
- CD Antigens
Description
| Product Name: | Mouse sCD40L Recombinant Protein |
| Product Code: | RPPB0895 |
| Size: | 25µg |
| Species: | Mouse |
| Target: | sCD40L |
| Synonyms: | CD40-L, Tumor necrosis factor ligand superfamily member 5, TNF-related activation protein, TRAP, T cell antigen Gp39, CD154 antigen, sCD40, IGM, IMD3, HIGM1, T-BAM, TNFSF5, hCD40L. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a sterile concentrated solution (1mg/ml) with 10mM Sodium Phosphate pH=7.5. |
| Solubility: | It is recommended to reconstitute the lyophilized sCD40 in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized sCD40 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CD154 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Arg-Gly-Asp |
| Biological Activity: | The ED50 as determined by its ability to induce MIP-1 alpha & TNF-alpha from mouse splenocytes was found to be 0.1�g/ml corresponding to a Specific Activity of 10,000IU/mg. |
CD40L or CD154 is a membrane glycoprotein and differentiation antigen expressed on the surface of T-cells. The CD40 ligand stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines . CD40 ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also costimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma , TNF-alpha , and IL2 .
sCD40 Mouse Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 149 amino acids and having a molecular mass of 16409 Dalton. The sCD40 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CD40LG: Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1); also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. Belongs to the tumor necrosis factor family. |
| UniProt Protein Details: | Protein type:Cell adhesion; Membrane protein, integral Cellular Component: extracellular space; cell surface; membrane; cell; integral to membrane; extracellular region; plasma membrane; external side of plasma membrane Molecular Function:CD40 receptor binding; cytokine activity; tumor necrosis factor receptor binding Biological Process: B cell proliferation; platelet activation; positive regulation of interleukin-12 production; isotype switching; positive regulation of immune response to tumor cell; signal transduction; positive regulation of interleukin-10 production; positive regulation of interferon-gamma production; positive regulation of MHC class II biosynthetic process; B cell differentiation; immunoglobulin secretion; positive regulation of B cell proliferation; immune response; inflammatory response; regulation of immunoglobulin secretion; negative regulation of apoptosis |
| UniProt Code: | P27548 |
| NCBI GenInfo Identifier: | 18314332 |
| NCBI Gene ID: | 21947 |
| NCBI Accession: | P27548.2 |
| UniProt Related Accession: | P27548 |
| Molecular Weight: | 29,370 Da |
| NCBI Full Name: | CD40 ligand |
| NCBI Synonym Full Names: | CD40 ligand |
| NCBI Official Symbol: | Cd40lg�� |
| NCBI Official Synonym Symbols: | IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5�� |
| NCBI Protein Information: | CD40 ligand; T-cell antigen Gp39; TNF-related activation protein; tumor necrosis factor ligand superfamily member 5 |
| UniProt Protein Name: | CD40 ligand |
| UniProt Synonym Protein Names: | T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD_antigen: CD154Cleaved into the following 2 chains:CD40 ligand, membrane form; CD40 ligand, soluble form |
| UniProt Gene Name: | Cd40lg�� |
| UniProt Entry Name: | CD40L_MOUSE |
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