Mouse sCD14 (Soluble Cluster of Differentiation 14) ELISA Kit
The Mouse SCD14 (Soluble Cluster of Differentiation 14) ELISA Kit is specifically designed for the accurate quantification of SCD14 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.SCD14 is a key protein involved in the immune response and inflammatory processes. It plays a critical role in innate immunity by binding to bacterial lipopolysaccharides, triggering immune responses and modulating inflammation.
The measurement of SCD14 levels is important for studying immune system function, inflammatory diseases, and infectious conditions in mouse models.With the Mouse SCD14 ELISA Kit, researchers can confidently measure SCD14 levels in biological samples, gaining valuable insights into immune responses and inflammatory pathways. This kit is a valuable tool for investigating various disease states and developing potential therapeutic interventions.
Product Name:
Mouse sCD14 (Soluble Cluster of Differentiation 14) ELISA Kit
SKU:
MOES01710
Target:
Mouse sCD14 (Soluble Cluster of Differentiation 14)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse sCD14. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse sCD14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse sCD14, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse sCD14. You can calculate the concentration of Mouse sCD14 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
101-111
96-110
Average (%)
99
106
103
1:4
Range (%)
89-103
81-90
83-96
Average (%)
94
86
88
1:8
Range (%)
87-100
79-95
87-99
Average (%)
92
86
93
1:16
Range (%)
90-103
83-96
85-96
Average (%)
96
88
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
97
EDTA plasma (n=5)
87-101
93
Cell culture media (n=5)
86-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.27
11.29
35.99
5.22
10.58
39.06
Standard deviation
0.35
0.58
1.1
0.3
0.48
1.27
C V (%)
6.64
5.14
3.06
5.75
4.54
3.25
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse sCD14 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse sCD14 in samples. No significant cross-reactivity or interference between Mouse sCD14 and analogues was observed.
