The Mouse SCCA2 (Squamous Cell Carcinoma Antigen 2) CLIA Kit is a powerful tool for the precise measurement of SCCA2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for a variety of research applications.SCCA2 is a key protein associated with squamous cell carcinoma, a type of skin cancer that can also affect other organs. Understanding SCCA2 levels can provide valuable insights into the progression and treatment of this aggressive cancer.
With its reliable performance, the Mouse SCCA2 CLIA Kit is an indispensable resource for studying cancer biology and developing novel therapeutic strategies.Don't miss out on the opportunity to explore the role of SCCA2 in cancer research – get your Mouse SCCA2 CLIA Kit today and start unraveling the mysteries of squamous cell carcinoma.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SCCA2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SCCA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SCCA2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse SCCA2. You can calculate the concentration of Mouse SCCA2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-110
86-98
99-112
Average (%)
101
92
107
1:4
Range (%)
96-110
86-98
102-116
Average (%)
103
93
109
1:8
Range (%)
87-100
99-113
92-109
Average (%)
93
107
100
1:16
Range (%)
86-96
84-99
86-97
Average (%)
91
90
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-106
97
EDTA plasma (n=5)
84-98
90
Cell culture media (n=5)
92-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.66
112.02
281.13
40.66
117.69
268.23
Standard deviation
3.51
11.43
29.57
4.92
13.99
23.77
C V (%)
8.43
10.2
10.52
12.1
11.89
8.86
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse SCCA2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SCCA2 in samples. No significant cross-reactivity or interference between Mouse SCCA2 and analogues was observed.
