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Mouse Scavenger Receptor Class A Member 3 (SCARA3) ELISA Kit

SKU:
MODL01153
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8C850
Sensitivity:
0.33ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
CSR1,CSR,MSLR1,APC7,MSRL1,Macrophage Scavenger Receptor-Like 1,Cellular stress response gene protein
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

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Mouse Scavenger Receptor Class A Member 3 (SCARA3) ELISA Kit

The Mouse SCARA3 (Scavenger Receptor Class A Member 3) ELISA Kit is a reliable and accurate tool for the detection of SCARA3 levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures trustworthy and reproducible results, making it a valuable asset for various research applications.SCARA3 is an important protein involved in scavenger receptor activity, playing a key role in the clearance of potentially harmful molecules in the body.

Dysregulation of SCARA3 has been linked to various diseases and conditions, making it a significant biomarker for studying these disorders and potentially developing new therapeutic interventions.Overall, the Mouse SCARA3 ELISA Kit provides researchers with a reliable and efficient way to quantify SCARA3 levels in mouse samples, allowing for in-depth investigation and understanding of its role in biological processes and disease pathogenesis.

Product Name:Mouse Scavenger Receptor Class A Member 3 (SCARA3) ELISA Kit
Product Code:MODL01153
Size:96 Assays
Target:SCARA3
Synonyms:CSR1, CSR, MSLR1, APC7, MSRL1, Macrophage Scavenger Receptor-Like 1, Cellular stress response gene protein
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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