Mouse sAPP alpha (soluble amyloid precursor protein alpha) ELISA Kit
The Mouse sAPP Alpha (Soluble Amyloid Precursor Protein Alpha) ELISA Kit is a powerful tool for measuring levels of sAPP Alpha in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.sAPP Alpha is a key protein in the processing of amyloid precursor protein and the pathogenesis of Alzheimer's disease.
Measurement of sAPP Alpha levels can provide valuable insights into the progression of neurodegenerative disorders and potential therapeutic targets.With its advanced technology and ease of use, the Mouse sAPP Alpha ELISA Kit is an essential tool for researchers studying Alzheimer's disease and related conditions. Trust Assay Genie for reliable results and innovative solutions in your research endeavors.
Product Name:
Mouse sAPP alpha (soluble amyloid precursor protein alpha) ELISA Kit
SKU:
MOES01808
Target:
Mouse sAPP alpha (soluble amyloid precursor protein alpha)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse sAPPα. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse sAPPα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse sAPPα, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse sAPPα. You can calculate the concentration of Mouse sAPPα in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-104
97-113
93-105
Average (%)
95
103
99
1:4
Range (%)
89-99
88-99
82-96
Average (%)
94
93
88
1:8
Range (%)
91-104
84-97
80-94
Average (%)
96
90
87
1:16
Range (%)
86-102
83-94
87-100
Average (%)
93
88
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
95
EDTA plasma (n=5)
94-109
101
Cell culture media (n=5)
92-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.15
24.28
95.07
9.42
24.05
98.89
Standard deviation
0.64
1.25
3.15
0.49
1.25
3.41
C V (%)
6.99
5.15
3.31
5.2
5.2
3.45
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse sAPPα concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse sAPPα in samples. No significant cross-reactivity or interference between Mouse sAPPα and analogues was observed.
