The Mouse Serum Amyloid A (SAA) ELISA Kit is a cutting-edge assay designed for the precise quantification of Serum Amyloid A protein levels in a variety of biological samples. Serum Amyloid A is an acute-phase protein primarily produced in response to pro-inflammatory stimuli, making it a crucial biomarker for monitoring inflammatory conditions, infectious diseases, and various pathological processes. Elevations in SAA levels are associated with a spectrum of inflammatory disorders, including rheumatoid arthritis, atherosclerosis, and certain cancers, highlighting its significance in disease diagnosis, prognosis, and treatment evaluation. This ELISA kit empowers researchers and clinicians to accurately measure SAA concentrations, providing valuable insights into the inflammatory status and disease progression.
By quantifying SAA levels with precision, scientists can gain a deeper understanding of its role in the acute-phase response and its implications in various inflammatory conditions, enabling tailored therapeutic interventions and personalized patient care. The SAA ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring reliable and reproducible results for translational research and clinical applications. Manufactured under stringent quality control standards, this kit delivers robust performance and user-friendly operation, making it the ideal choice for investigating the role of Serum Amyloid A in health and disease pathways with high confidence and accuracy.
Product Name:
Mouse SAA (Serum Amyloid A) ELISA Kit
SKU:
AEES00238
Target:
Mouse SAA (Serum Amyloid A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SAA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SAA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SAA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse SAA. You can calculate the concentration of Mouse SAA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-109
87-100
95-106
Average (%)
104
92
102
1:4
Range (%)
89-97
88-95
82-94
Average (%)
93
91
90
1:8
Range (%)
98-108
85-93
98-104
Average (%)
105
90
101
1:16
Range (%)
95-103
90-101
92-99
Average (%)
100
94
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-109
105
EDTA plasma (n=5)
93-107
101
Cell culture media (n=5)
92-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
320
903.33
3234.73
338.06
911.72
3357.81
Standard deviation
16.32
43.27
138.77
16.94
44.58
141.36
C V (%)
5.1
4.79
4.29
5.01
4.89
4.21
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse SAA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SAA in samples. No significant cross-reactivity or interference between Mouse SAA and analogues was observed.
