Mouse S100B (S100 Calcium Binding Protein B) CLIA Kit
The Mouse S100B (S100 Calcium Binding Protein B) CLIA Kit is a reliable tool for measuring the levels of S100B in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and consistent results, making it an excellent choice for various research applications.S100B is a calcium-binding protein that is involved in regulating cell growth, differentiation, and inflammation. It plays a crucial role in various physiological processes and has been linked to conditions such as Alzheimer's disease, stroke, and traumatic brain injury.
By measuring S100B levels, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially develop new therapeutic approaches.Overall, the Mouse S100B CLIA Kit from AssayGenie is a valuable tool for scientists studying neurodegenerative disorders and inflammatory diseases in mouse models, offering precise measurements and reliable data for their research projects.
Product Name:
Mouse S100B (S100 Calcium Binding Protein B) CLIA Kit
SKU:
MOES00522
Target:
Mouse S100B (S100 Calcium Binding Protein B)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse S100B. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse S100B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse S100B, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse S100B. You can calculate the concentration of Mouse S100B in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-102
101-119
97-109
Average (%)
96
109
103
1:4
Range (%)
89-100
94-110
90-102
Average (%)
94
101
97
1:8
Range (%)
87-100
99-115
94-108
Average (%)
94
105
100
1:16
Range (%)
92-104
91-106
92-108
Average (%)
99
98
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
93
EDTA plasma (n=5)
91-107
98
Cell culture media (n=5)
90-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.77
92.47
404.92
45.73
93.75
431.28
Standard deviation
4.6
9.92
34.58
4.5
11.24
47.57
C V (%)
9.06
10.73
8.54
9.84
11.99
11.03
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse S100B concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse S100B in samples. No significant cross-reactivity or interference between Mouse S100B and analogues was observed.
