Mouse S100A9 (S100 Calcium Binding Protein A9) ELISA Kit
The Mouse S100 Calcium Binding Protein A9 (S100A9) ELISA Kit is a specialized assay designed for the precise quantitative analysis of S100A9 levels in a variety of biological samples. S100A9, a member of the S100 protein family, is a calcium-binding protein known for its involvement in various biological processes, including inflammation, immune response regulation, and cell differentiation. Elevated levels of S100A9 are often associated with inflammatory conditions, autoimmune disorders, and cancer progression, making it a vital protein of interest in diverse research areas. Accurate measurement of S100A9 is essential for unraveling its role in modulating immune responses, understanding disease pathogenesis, and potentially identifying diagnostic or therapeutic targets. By quantifying S100A9 levels in biological samples, researchers can gain valuable insights into the protein's involvement in inflammatory pathways, cell signaling mechanisms, and disease progression.
This quantitative data is pivotal for advancing our knowledge of S100A9 biology and its implications in various health conditions. The Mouse S100A9 ELISA Kit from Assay Genie offers high sensitivity, specificity, and reproducibility, ensuring dependable and consistent results for your research needs. Manufactured under rigorous quality control standards, this kit provides robust performance and user-friendly protocols, making it an ideal choice for scientists investigating the functional roles and clinical significance of S100A9. Choose Assay Genie's S100A9 ELISA Kit for accurate quantification of S100A9 levels and uncover the multifaceted roles of S100A9 in inflammation, immunity, and disease processes.
Product Name:
Mouse S100A9 (S100 Calcium Binding Protein A9) ELISA Kit
SKU:
AEES00242
Target:
Mouse S100A9 (S100 Calcium Binding Protein A9)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse S100A9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse S100A9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse S100A9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse S100A9. You can calculate the concentration of Mouse S100A9 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-109
94-107
96-105
Average (%)
104
101
101
1:4
Range (%)
95-106
89-98
87-98
Average (%)
100
95
92
1:8
Range (%)
96-106
90-100
87-95
Average (%)
100
96
92
1:16
Range (%)
89-101
86-95
87-101
Average (%)
95
90
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-107
101
EDTA plasma (n=5)
102-111
105
Cell culture media (n=5)
97-110
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.74
4.96
16.68
1.64
4.24
17.5
Standard deviation
0.1
0.22
0.75
0.09
0.19
0.83
C V (%)
5.75
4.44
4.5
5.49
4.48
4.74
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse S100A9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse S100A9 in samples. No significant cross-reactivity or interference between Mouse S100A9 and analogues was observed.
