Mouse S100A8 (S100 Calcium Binding Protein A8) ELISA Kit
The Mouse S100 Calcium Binding Protein A8 (S100A8) ELISA Kit is a specialized assay crafted to quantitatively analyze the levels of S100A8 in diverse biological samples with precision and high sensitivity.
S100A8, a member of the S100 protein family, is a calcium-binding protein known for its involvement in various cellular processes, including inflammation, immune response modulation, and cell differentiation. High levels of S100A8 are often linked to inflammatory conditions, autoimmune diseases, cancer progression, and other pathological states, making it a crucial protein of interest in biomedical research. By accurately measuring S100A8 levels, researchers can unravel its intricate roles in inflammation, immunity, and disease pathogenesis, providing valuable insights into potential biomarkers and therapeutic targets. Understanding the functions and regulation of S100A8 is essential for deciphering its impact on cellular dynamics and its contribution to disease development, offering new avenues for therapeutic interventions and diagnostic strategies. The Mouse S100A8 ELISA Kit from Assay Genie is meticulously designed to deliver exceptional sensitivity and specificity, ensuring robust and reproducible results for your investigative studies. Developed under strict quality control measures, this kit guarantees reliable performance and user-friendly procedures, making it a trusted tool for researchers investigating the biological roles of S100A8 in health and disease. Choose Assay Genie's S100A8 ELISA Kit for precise quantification of S100A8 levels and illuminate the complexity of S100A8-mediated processes in diverse biological contexts, ranging from inflammation to cancer research and beyond.
Product Name:
Mouse S100A8 (S100 Calcium Binding Protein A8) ELISA Kit
SKU:
AEES00241
Target:
Mouse S100A8 (S100 Calcium Binding Protein A8)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse S100A8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse S100A8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse S100A8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse S100A8. You can calculate the concentration of Mouse S100A8 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-106
91-105
99-105
Average (%)
103
98
102
1:4
Range (%)
95-108
97-107
85-96
Average (%)
101
100
90
1:8
Range (%)
101-113
97-110
97-107
Average (%)
105
102
104
1:16
Range (%)
87-98
96-106
96-111
Average (%)
90
99
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
93
EDTA plasma (n=5)
83-95
90
Cell culture media (n=5)
90-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
188.64
414.53
1934.43
195.77
446.65
1946.95
Standard deviation
11.11
20.6
92.08
11.39
20.64
78.07
C V (%)
5.89
4.97
4.76
5.82
4.62
4.01
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse S100A8 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse S100A8 in samples. No significant cross-reactivity or interference between Mouse S100A8 and analogues was observed.
