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Mouse Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit

SKU:
MODL01139
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
E9PZQ0
Sensitivity:
0.072ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CCO,MHS,MHS1,RYDR,RYR,SKRR,Central Core Disease Of Muscle,Skeletal muscle calcium release channel
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit

The Mouse Ryanodine Receptor 1 (Skeletal) (RYR1) ELISA Kit is specifically designed for the accurate detection of RYR1 levels in mouse serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring precise and reproducible results for a variety of research applications.RYR1 is a critical protein involved in skeletal muscle function, playing a key role in muscle contraction and calcium release. Dysregulation of RYR1 has been linked to various muscle disorders and diseases, making it a valuable biomarker for studying these conditions and developing potential therapies.

With the Mouse RYR1 ELISA Kit, researchers can confidently measure RYR1 levels in mouse samples, gaining insights into skeletal muscle function and potential therapeutic targets. This kit is a valuable tool for advancing research in muscle-related diseases and disorders.

Product Name:Mouse Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
Product Code:MODL01139
Size:96 Assays
Target:RYR1
Synonyms:CCO, MHS, MHS1, RYDR, RYR, SKRR, Central Core Disease Of Muscle, Skeletal muscle calcium release channel
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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