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Mouse RISC-loading complex subunit TARBP2 (Tarbp2) ELISA Kit (MOEB2163)

SKU:
MOEB2163
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P97473
ELISA Type:
Sandwich
Reactivity:
Mouse
€649
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Description

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Product Name:Mouse RISC-loading complex subunit TARBP2 (Tarbp2) ELISA Kit
SKU:MOEB2163
Size:96T
Target:Mouse RISC-loading complex subunit TARBP2 (Tarbp2)
Synonyms:Protamine-1 RNA-binding protein, TAR RNA-binding protein 2, PRM-1 RNA-binding protein, Prbp
Assay Type:Sandwich
Detection Method:ELISA
Reactivity:Mouse
Detection Range:0.312-20ng/mL
Sensitivity:0.156ng/mL
Intra CV:4.7%
Inter CV:6.4%
Linearity:
Sample1:21:41:81:16
Serum(N=5)95-105%115-125%106-115%104-116%
EDTA Plasma(N=5)97-105%81-89%119-119%94-103%
Heparin Plasma(N=5)100-112%99-109%89-99%103-112%
Recovery:
Sample TypeAverage(%)Recovery Range(%)
Serum9993-105
Plasma10195-107
Function:Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1 (By similarity). Binds in vitro to the PRM1 3'-UTR. Seems to act as a repressor of translation (PubMed:8649414).
Uniprot:P97473
Sample Type:Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Specificity:Natural and recombinant mouse RISC-loading complex subunit TARBP2
Sub Unit:Self-associates. Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Note that the trimeric RLC/miRLC is also referred to as RISC. Interacts with EIF2AK2/PKR and inhibits its protein kinase activity. Interacts with DHX9 (By similarity). Interacts with DICER1 and PRKRA (PubMed:16142218, PubMed:17452327). Interacts with DICER1, AGO2, MOV10, EIF6 and RPL7A (60S ribosome subunit); they form a large RNA-induced silencing complex (RISC).
Subcellular Location:Cytoplasm Cytoplasm Perinuclear region Nucleus
Storage:Please see kit components below for exact storage details
Note:For research use only
UniProt Protein Function:TRBP: the HIV-1 TAR RNA binding protein (TRBP) is a double strand RNA binding protein (dsRBP) that is required for the recruitment of Ago2 to the small interfering RNA (siRNA) bound by Dicer. TRBP increases HIV-1 expression and replication by inhibiting the interferon-induced protein kinase PKR and by increasing translation of viral mRNA. Dicer, Ago2, and TRBP are the main components of the RNA-induced silencing complex (RISC).Protein type: Motility/polarity/chemotaxis; Translation; RNA processing; RNA-bindingCellular Component: nucleoplasm; cytoplasm; nucleusMolecular Function: protein binding; siRNA binding; protein homodimerization activity; RNA binding; double-stranded RNA bindingBiological Process: positive regulation of viral genome replication; positive regulation of translation; multicellular organism growth; RNA interference, targeting of mRNA for destruction; miRNA-mediated gene silencing, miRNA loading onto RISC; negative regulation of defense response to virus by host; regulation of translation; regulation of viral transcription; pre-microRNA processing; single fertilization; RNA-mediated gene silencing; RNA interference, production of siRNA; spermatid development
UniProt Protein Details:
NCBI Summary:
UniProt Code:P97473
NCBI GenInfo Identifier:166295185
NCBI Gene ID:21357
NCBI Accession:NP_033345.2
UniProt Secondary Accession:P97473,Q99M41
UniProt Related Accession:P97473
Molecular Weight:38,842 Da
NCBI Full Name:RISC-loading complex subunit TARBP2 isoform 1
NCBI Synonym Full Names:TAR (HIV) RNA binding protein 2
NCBI Official Symbol:Tarbp2
NCBI Official Synonym Symbols:Prbp; TRBP
NCBI Protein Information:RISC-loading complex subunit TARBP2
UniProt Protein Name:RISC-loading complex subunit TARBP2
UniProt Synonym Protein Names:Protamine-1 RNA-binding protein; PRM-1 RNA-binding protein; TAR RNA-binding protein 2
Protein Family:RISC-loading complex
UniProt Gene Name:Tarbp2
UniProt Entry Name:TRBP2_MOUSE
Component Quantity (96 Assays) Storage
ELISA Microplate (Dismountable) 8×12 strips -20°C
Lyophilized Standard 2 -20°C
Sample Diluent 20ml -20°C
Assay Diluent A 10mL -20°C
Assay Diluent B 10mL -20°C
Detection Reagent A 120µL -20°C
Detection Reagent B 120µL -20°C
Wash Buffer 30mL 4°C
Substrate 10mL 4°C
Stop Solution 10mL 4°C
Plate Sealer 5 -

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir

*Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Allow all reagents to reach room temperature (Please do not dissolve the reagents at 37°C directly). All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. Keep appropriate numbers of strips for 1 experiment and remove extra strips from microtiter plate. Removed strips should be resealed and stored at -20°C until the kits expiry date. Prepare all reagents, working standards and samples as directed in the previous sections. Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their experiments. We recommend running all samples in duplicate.

Step
1. Add Sample: Add 100µL of Standard, Blank, or Sample per well. The blank well is added with Sample diluent. Solutions are added to the bottom of micro ELISA plate well, avoid inside wall touching and foaming as possible. Mix it gently. Cover the plate with sealer we provided. Incubate for 120 minutes at 37°C.
2. Remove the liquid from each well, don't wash. Add 100µL of Detection Reagent A working solution to each well. Cover with the Plate sealer. Gently tap the plate to ensure thorough mixing. Incubate for 1 hour at 37°C. Note: if Detection Reagent A appears cloudy warm to room temperature until solution is uniform.
3. Aspirate each well and wash, repeating the process three times. Wash by filling each well with Wash Buffer (approximately 400µL) (a squirt bottle, multi-channel pipette,manifold dispenser or automated washer are needed). Complete removal of liquid at each step is essential. After the last wash, completely remove remaining Wash Buffer by aspirating or decanting. Invert the plate and pat it against thick clean absorbent paper.
4. Add 100µL of Detection Reagent B working solution to each well. Cover with the Plate sealer. Incubate for 60 minutes at 37°C.
5. Repeat the wash process for five times as conducted in step 3.
6. Add 90µL of Substrate Solution to each well. Cover with a new Plate sealer and incubate for 10-20 minutes at 37°C. Protect the plate from light. The reaction time can be shortened or extended according to the actual color change, but this should not exceed more than 30 minutes. When apparent gradient appears in standard wells, user should terminatethe reaction.
7. Add 50µL of Stop Solution to each well. If color change does not appear uniform, gently tap the plate to ensure thorough mixing.
8. Determine the optical density (OD value) of each well at once, using a micro-plate reader set to 450 nm. User should open the micro-plate reader in advance, preheat the instrument, and set the testing parameters.
9. After experiment, store all reagents according to the specified storage temperature respectively until their expiry.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample Type Protocol
Serum If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.
Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal Fluid Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatant Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysates Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenates The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysates Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast Milk Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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