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Mouse Ribonucleotide Reductase M1 (RRM1) ELISA Kit

SKU:
MODL01135
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P07742
Sensitivity:
0.124ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
R1,RIR1,RR1,Ribonucleoside-diphosphate reductase large subunit
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Ribonucleotide Reductase M1 (RRM1) ELISA Kit

The Mouse Ribonucleotide Reductase M1 (RRM1) ELISA Kit is a reliable tool for measuring levels of RRM1 in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it suitable for a variety of research applications.RRM1 is an essential enzyme involved in DNA synthesis and repair, making it a critical component in cell proliferation and survival. Dysregulation of RRM1 expression has been implicated in cancer and other diseases, highlighting its potential as a biomarker for disease diagnosis and treatment evaluation.

This ELISA kit provides researchers with a valuable tool for studying RRM1 expression in mouse models, offering insights into the role of this enzyme in various biological processes and disease states. With its user-friendly protocol and reliable performance, the Mouse Ribonucleotide Reductase M1 ELISA Kit is a must-have for any laboratory conducting research in this field.

Product Name:Mouse Ribonucleotide Reductase M1 (RRM1) ELISA Kit
Product Code:MODL01135
Size:96 Assays
Target:RRM1
Synonyms:R1, RIR1, RR1, Ribonucleoside-diphosphate reductase large subunit
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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