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Mouse Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) ELISA Kit

SKU:
MODL00113
Product Type:
ELISA Kit
Sensitivity:
34pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
PAK3,BETA-PIX,COOL1,P50,P50BP,P85,P85SPR,PIXB,SH3 Domain-Containing Proline-Rich Protein,PAK-Interacting Exchange Factor Beta
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

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Mouse Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) ELISA Kit

The Mouse Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) ELISA Kit is specifically designed for the precise quantification of ARHGEF7 levels in mouse serum, plasma, and cell culture supernatants. This ELISA kit offers high sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.ARHGEF7 is a key protein involved in the regulation of Rho GTPases, which play a crucial role in cell migration, adhesion, and polarity. Dysregulation of Rho GTPases has been implicated in various diseases such as cancer, cardiovascular disorders, and immune disorders.

Therefore, the ARHGEF7 ELISA kit is an essential tool for researchers studying these conditions and exploring potential therapeutic interventions.Overall, the Mouse Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) ELISA Kit is a reliable and efficient assay for investigating the role of ARHGEF7 in physiological and pathological processes, providing valuable insights for basic research and drug development efforts.

Product Name:Mouse Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) ELISA Kit
Product Code:MODL00113
Size:96 Assays
Target:ARHGEF7
Synonyms:PAK3, BETA-PIX, COOL1, P50, P50BP, P85, P85SPR, PIXB, SH3 Domain-Containing Proline-Rich Protein, PAK-Interacting Exchange Factor Beta
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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