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Mouse RBP4 (Retinol Binding Protein 4) ELISA Kit (AEES00236)

SKU:
AEES00236
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Mouse
Sensitivity:
0.38 ng/mL
Range:
0.63-40 ng/mL
Sample Type:
Serum, plasma and other biological fluids
$599
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Description

Mouse RBP4 (Retinol Binding Protein 4) ELISA Kit

The Mouse Retinol Binding Protein 4 (RBP4) ELISA Kit is a specialized assay designed to accurately quantify RBP4 levels in various biological samples. RBP4 is a transporter protein that plays a crucial role in the delivery of retinol to tissues and regulates the circulating levels of vitamin A. Dysregulation of RBP4 has been linked to metabolic disorders such as insulin resistance, obesity, and diabetes, making it a valuable biomarker in metabolic research. This ELISA kit enables researchers to investigate the concentrations of RBP4, offering insights into its involvement in metabolic pathways and the development of associated diseases.

By accurately measuring RBP4 levels, scientists can better understand the pathophysiological mechanisms underlying metabolic conditions and explore therapeutic strategies aimed at modulating RBP4 expression. The RBP4 ELISA Kit from Assay Genie provides outstanding sensitivity and specificity, ensuring precise and reproducible results. With stringent quality control measures in place, this kit delivers reliable performance and ease of use, making it an ideal choice for cutting-edge research in the field of metabolic disorders and vitamin A metabolism.

Product Name:Mouse RBP4 (Retinol Binding Protein 4) ELISA Kit
Product Code:AEES00236
Assay Type:Sandwich
Format:96T
Assay Time:3.5h
Reactivity:Mouse
Detection Range:0.63-40 ng/mL
Sensitivity:0.38 ng/mL
Sample Type & Sample Volume:Serum, plasma and other biological fluids, 100μL
Specificity:This kit recognizes Mouse RBP4 in samples. No significant cross-reactivity or interference between Mouse RBP4 and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Mouse RBP4 concentrations in serum, plasma and other biological fluids.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse RBP4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse RBP4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse RBP4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse RBP4. You can calculate the concentration of Mouse RBP4 in the samples by comparing the OD of the samples to the standard curve.

Kit Components:

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells x 12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8℃(Protect from light)
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Manual1 copy
Certificate of Analysis1 copy
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
3. Aspirate and wash the plate for 3 times
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
6. Add 50μL Stop Solution
7. Read the plate at 450nm immediately. Calculation of the results

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