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Mouse Pyruvate Kinase, Muscle (PKM2) ELISA Kit

SKU:
MODL01055
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P52480
Sensitivity:
0.144ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
M2PK,PKM,M2-PK,PKM1,CTHBP,OIP3,PK3,PKM,TCB,THBP1,Pyruvate kinase muscle isozyme,Thyroid hormone-binding protein 1,Cytosolic thyroid hormone-binding protein
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Pyruvate Kinase, Muscle (PKM2) ELISA Kit

The Mouse Pyruvate Kinase Muscle (PKM2) ELISA Kit is specifically designed for accurate detection of PKM2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a wide range of research applications.PKM2 is a key enzyme involved in glycolysis and is known to play a crucial role in regulating cellular metabolism and energy production.

Dysregulation of PKM2 has been associated with various diseases, including cancer and metabolic disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.With its user-friendly protocol and robust performance, the Mouse Pyruvate Kinase Muscle (PKM2) ELISA Kit is an essential tool for researchers investigating the role of PKM2 in health and disease.

Product Name:Mouse Pyruvate Kinase, Muscle (PKM2) ELISA Kit
Product Code:MODL01055
Size:96 Assays
Target:PKM2
Synonyms:M2PK, PKM, M2-PK, PKM1, CTHBP, OIP3, PK3, PKM, TCB, THBP1, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, Cytosolic thyroid hormone-binding protein
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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